K-BALB (K-234) (ATCC® CCL-163.3)

Organism: Mus musculus, mouse  /  Cell Type:: fibroblastKirsten murine sarcoma virus (Ki-MSV) transformed  / 

Organism Mus musculus, mouse
Cell Type fibroblastKirsten murine sarcoma virus (Ki-MSV) transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 14 to 17 days gestation embryo
Strain BALB/c
Applications
They do not produce Ki-MSV.
Antigen Expression
H-2d
Genes Expressed
H-2d
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
They do not produce Ki-MSV.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Aaronson
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769

Basic Documentation
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769