PC-12 Adh (ATCC® CRL-1721.1)

Organism: Rattus norvegicus, rat  /  Tissue: adrenal gland  /  Disease: pheochromocytoma

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Organism Rattus norvegicus, rat
Tissue adrenal gland
Product Format frozen
Morphology polygonal
Culture Properties adherent, small clusters
Biosafety Level 1
Disease pheochromocytoma
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype 40 chromosomes; 38 autosomes plus XY RefGreene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
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Derivation
The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
Clinical Data
male
Genes Expressed
catecholamines; dopamine; norepinephrine
RefGreene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
Tumorigenic Yes
Effects
Yes, in New England Deaconess Hospital strain rats
Comments
The cells do not synthesize epinephrine. This adherent variant (PC-12 Adh) has been adapted to Corning CellBIND® flasks to improve cell attachment.
ATCC has not tested CRL-1721.1 cells for differentiation potential in response to NGF treatment. Please refer to the ATCC CRL-1721 cell line which responds reversibly to NGF by induction of the neuronal phenotype.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing

Use Corning CellBIND flasks (Corning catalog #3289 through 3293)
Volumes used for this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard old culture medium.
  2. Pipet 10 mL fresh medium over the cell sheet and scrape.
  3. Aspirate cells with a small bore pipette to break up clusters.
  4. Add appropriate aliquots of the cell suspension to new Corning CellBIND® 75 cm2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 104 to 3.0 x 104 viable cells / cm2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 105 to 2.0 x 105 viable cells/cm2.
  5. Place culture vessels in incubator at 37°C.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Population Doubling Time 48 hrs
Name of Depositor B Patterson
References

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897