L8 ATCC ® CRL-1769™ Rattus norvegicus Carcinogen

Permits and Restrictions	View Permits
Organism	Rattus norvegicus, rat
Cell Type	myoblast myoblast
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Carcinogen
Age	newborn
Applications	transfection host

Derivation	This line was originally isolated by D. Yaffe in 1969 from primary rat skeletal muscle cultures;
Genes Expressed	creatine phosphokinase (CPK); myosin
Cellular Products	creatine phosphokinase (CPK); myosin
Comments	This line was originally isolated by D. Yaffe in 1969 from primary rat skeletal muscle cultures; unlike the L6 cell line (ATCC CRL-1458) no carcinogen was used to establish the L8 line; upon becoming confluent, L8 will fuse to form cross striated multinucleated muscle fibers; it is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse; it is important that the cells be subcultured when the flask is about 60% confluent. The myoblastic population will be depleted if the cultures are allowed to become confluent since most of the cells will fuse into nondividing syncytia. To avoid this, one must subculture before before the cultures become confluent and should reclone periodically and select myoblastic clones.

Complete Growth Medium	A 4:1 mixture of Dulbecco's modified Eagle's medium and Medium 199, 89%; chicken embryo extract, 1%; horse serum, 10%
Subculturing	Subcultivation Ratio: An inoculation density of 4000 viable cells per sq. cm. of flask or dish surface area is recommended Medium Renewal: Twice per week Remove medium, rinse the cell sheet once with fresh trypsin (0.25%), EDTA (0.03%) solution, remove trypsin and allow the flask to sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks at a density of 2000 cells/sq. cm.
Cryopreservation	Culture medium, 95%; DMSO, 5%

Name of Depositor	B Paterson
Deposited As	Rattus sp.
Passage History	it is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse;
References	Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965 Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
References	Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965 Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

References

Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965

Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123

L8 (ATCC^® CRL-1769^™)