G-7 (ATCC® CRL-1447)

Organism: Mus musculus, mouse  /  Cell Type: myoblast myoblast  / 

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type myoblast myoblast
Product Format frozen
Morphology myoblast
Culture Properties adherent
Biosafety Level 1
Age fetus fetus
Strain Swiss
Applications
They are slightly responsive to acetylcholine.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Karyotype modal number = 40
Tumorigenic Yes
Effects
Yes,
Comments
When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 17 to 19 hrs
Name of Depositor J Peacock
References

Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191