Complete Growth Medium
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The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing
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Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended Medium Renewal: 2 to 3 times per week To dislodge adherent cells, remove all but 5 to 10 ml of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
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