NCI-H64 [H64] (ATCC® CRL-5976)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: stage E, carcinoma; small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Product Format frozen
Culture Properties floating aggregates of round cell clusters
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease stage E, carcinoma; small cell lung cancer
Age 48 years
Gender female
Ethnicity Caucasian
The line was established in May 1977.
Karyotype 3p is normal
The small cell lung carcinoma (SCLC) line was established in May 1977.
Clinical Data
48 years
The line was established in May 1977.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
30 pack years.
Complete Growth Medium HITES medium supplemented with 20% fetal bovine serum
The base medium for this cell line is DMEM:F12 Medium (ATCC Catalog No. 30-2006)
    To make the complete growth medium, add the following components to the base medium:
  1. Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
  2. Insulin 0.005 mg/ml
  3. Transferrin 0.01 mg/ml
  4. Sodium selenite 30 nM
  5. Hydrocortisone 10 nM
  6. beta-estradiol 10 nM
  7. HEPES 10 mM
  8. L-glutamine 2 mM (in addition to that in the base medium)
  9. fetal bovine serum 20%

Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 9,12
D5S818: 11
D7S820: 10,12
THO1: 6
vWA: 17,18
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1977

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI).  For further information, please contact NCI’s Technology Transfer Center at or by phone at (240)-276-5514.


NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.