NCI-H748 [H748] (ATCC® CRL-5841)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: lymph node  /  Disease: stage E, carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease stage E, carcinoma; classic small cell lung cancer
Age 62 years
Gender male
Ethnicity Black
Applications
The line was established in July 1984.
Karyotype t(1;3)del(3)(p14-p23)
Derivation
The line was established in July 1984.
Clinical Data
male
Black
62 years
Comments
The line was established in July 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
50.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12
D5S818: 10
D7S820: 10,11
THO1: 7,9
TPOX: 10,12
vWA: 17,19
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.