SU-DHL-5 (ATCC® CRL-2958)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: lymph node; derived from metastatic site: peritoneal effusion  /  Disease: large cell lymphoma; diffuse mixed histiocytic and lymphoma

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Organism Homo sapiens, human
Tissue lymph node; derived from metastatic site: peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension, multicell aggregates and single cells
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease large cell lymphoma; diffuse mixed histiocytic and lymphoma
Gender Female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2
Clinical Data 17 years
Antigen Expression CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38-, cyCD79a+, CD80-, HLA-DR +, sm/cyIg-, sm/cyIgM+, sm/cykappa-, sm/cylamb

Receptor Expression Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Comments The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
This cell line is negative for Candida ingestion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 104 to 1 X 105 viable cells/ mL. Maintain cultures at a cell concentration below 5 X 105 viable cells/ mL.
Medium renewal: Two to three times weekly
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: Culture can be maintained by the addition of fresh medium or re-seeding a new flask with cells in culture.
STR Profile
D5S818: 12, 15
D13S317: 12
D7S820: 10
D16S539: 11, 13
vWA: 17
TH01: 6, 9
Amelogenin: X
CSF1PO: 11, 13
Name of Depositor A Epstein
Year of Origin 1978
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation