SU-DHL-2 (ATCC® CRL-2956)

Organism: Homo sapiens, human  /  Cell Type: histiocytic  /  Tissue: lymph node
Derived from Metastatic Site: peritoneal effusion
 /  Disease: large cell lymphoma; diffuse histiocytic lymphoma

Organism Homo sapiens, human
Tissue lymph node
Derived from Metastatic Site: peritoneal effusion
Cell Type histiocytic
Product Format frozen
Morphology Lymphoblast-like
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease large cell lymphoma; diffuse histiocytic lymphoma
Age 73 years
Gender Female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Most of these cells were hyperdiploid, with a sharp modal number of 51 chromosomes; occasional cells in the hypertetraploid range were also seen. A minute marker chromosome was consistently observed, and increased numbers of chromosomes were seen in the A, B, C, and F groups.
Images CRL-2956 Cell Micrograph
Clinical Data 73 years
Caucasian
Female
Comments The cells are non-specific esterase and acid phosphatase positive.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
The cells are surface Ig negative (sIG-).
The cells are reported to be E - rosette negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 13
D13S317: 11, 12
D7S820: 11, 13
D16S539: 14
vWA: 17
THO1: 6, 9
TPOX: 8
CSF1PO: 12
Amelogenin: X
Name of Depositor A Epstein
Year of Origin 1974
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Basic Documentation
Other Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794