SVEC4-10EHR1 (ATCC® CRL-2161)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

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Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender male
Strain C3H/HeJ
Applications
SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
SVEC4-10EHR1 expresses the cell surface major histocompatibility complex class I antigen (H-2 k) of the parental cell line, and express the vascular cell adhesion molecule (VCAM).
Derivation
SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2.
Clinical Data
male
Antigen Expression
H-2 K; VCAM
Genes Expressed
H-2 K; VCAM
Tumorigenic Yes
Effects
Yes, the cells induced nodular tumors in nude mice with some histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments
SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2.
The cell line is distinguished by its spindle cell morphology both in vitro and in vivo in contrast to SVEC4-10EE2.
SVEC4-10EHR1 expresses the cell surface major histocompatibility complex class I antigen (H-2 k) of the parental cell line, and express the vascular cell adhesion molecule (VCAM).
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor KA O'Connell
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299