Hs 913T (ATCC® HTB-152)

Organism: Homo sapiens, human  /  Tissue: Derived from metastatic site: lung  /  Disease: fibrosarcoma

Organism Homo sapiens, human
Tissue Derived from metastatic site: lung
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease fibrosarcoma
Age 51 years
Gender male
Ethnicity Caucasian
Karyotype modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers
Clinical Data
51 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 10
D7S820: 10
THO1: 8,9.3
TPOX: 9,11
vWA: 17,18
AK-1, 1
ES-D, 1
GLO-I, 1
PGM1, 2
PGM3, 2
Name of Depositor RB Owens