NCI-H735 [H735] (ATCC® CRL-5978™) Organism: Homo sapiens, human / Tissue: lung; derived from metastatic site: liver / Disease: stage E, carcinoma; small cell lung cancer General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue lung; derived from metastatic site: liver Product Format frozen Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease stage E, carcinoma; small cell lung cancer Age 58 years Gender female Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Derivation The line was established in August 1984. Clinical Data The patient received prior chemotherapy.The patient was a smoker.femaleCaucasian58 years Complete Growth Medium HITES medium (serum free) HITES medium is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM beta-estradiol 10 nM HEPES 10 mM L-glutamine 2 mM (in addition to that in the base medium) Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% FBS and 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase Culture Conditions Temperature: 37°C Atmosphere: 5% CO2 in air. STR Profile Amelogenin: XCSF1PO: 12,14D13S317: 11,14D16S539: 14D5S818: 9,11D7S820: 8,11THO1: 8,9.3TPOX: 11vWA: 18,19 Year of Origin 1984 References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Restrictions The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.