NCI-H1563 [H1563] (ATCC® CRL-5875™) Organism: Homo sapiens, human / Tissue: lung / Disease: adenocarcinoma; non-small cell lung cancer General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue lung Product Format frozen Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease adenocarcinoma; non-small cell lung cancer Gender male Derivation The line was established in December 1986 from lung adenocarcinoma. Clinical Data maleThe tissue donor was a non-smoker. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Culture medium, 95%; DMSO, 5% Culture Conditions Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 10,11D13S317: 9,14D16S539: 9,13D5S818: 12,13D7S820: 7,8F13A01: 5,7F13B: 10FESFPS: 11LPL: 11THO1: 6TPOX: 8,11vWA: 17,18 Name of Depositor AF Gazdar, JD Minna Year of Origin 1986 References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Restrictions The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.