|
Karyotype
|
modal number = 72; range = 70 to 78
|
|
Derivation
|
The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung. Derived from metastatic site, lung
|
|
Clinical Data
|
The patient had not received prior radiation or chemotherapy. 62 years Caucasian male The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
|
|
Oncogene
|
c-myc +; v-src +; v-abl +; v-erb B +; c-raf 1 +; H-ras +; K-ras +; N-ras +; N-myc -; L-myc -; c-myb -; c-fos -; v-fes -; v-fms -; v-sis -
|
|
Effects
|
Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
|
|
Comments
|
The patient had not received prior radiation or chemotherapy.
MSTO-211H cells have high affinity binding sites for EGF, and express Neuron specific enolase (NSE) and both the alpha and beta subunits of human chorionic gonadotropin (HCG).
L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
The cells overexpress the c-myc protooncogene, and no gene rearrangement or amplification was observed.
They are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras.
Expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis oncogene expression was not detected.
The cells can reach a saturation density of 400000 cells per sq cm, but will slough off of the surface as they attain this density.
|