Dede (ATCC® CCL-39)

Organism: Cricetulus griseus, hamster, Chinese  / 

Permits and Restrictions

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Clinical Data
female
Virus Resistance
poliovirus 1
Complete Growth Medium McCoy’s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and allow culture to sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: If necessary, the serum can be reduced to 10%.
Name of Depositor TC Hsu
References

Stich HF, et al. Viruses and mammalian chromosomes. I. Localization of chromosome aberrations after infection with Herpes simplex virus. Virology 22: 439-445, 1964. PubMed: 14171598

Rapp F, Hsu TC. Viruses and mammalian chromosomes. IV. Replication of Herpes simplex virus in diploid Chinese hamster cells. Virology 25: 401-411, 1965. PubMed: 14328610

Stich HF, et al. Viruses and mammalian chromosomes; chromosome aberrations by human adenovirus type 12. Exp. Cell Res. 34: 400-403, 1964. PubMed: 14165598

Cross References

Nucleotide (GenBank) : X86458 C.griseus mRNA for serotonin receptor 1B.

Nucleotide (GenBank) : X61958 C.longicaudatus mRNA for thrombin receptor.

Nucleotide (GenBank) : X53791 Chinese hamster mRNA for 5-HT type 2 serotonin receptor.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stich HF, et al. Viruses and mammalian chromosomes. I. Localization of chromosome aberrations after infection with Herpes simplex virus. Virology 22: 439-445, 1964. PubMed: 14171598

Rapp F, Hsu TC. Viruses and mammalian chromosomes. IV. Replication of Herpes simplex virus in diploid Chinese hamster cells. Virology 25: 401-411, 1965. PubMed: 14328610

Stich HF, et al. Viruses and mammalian chromosomes; chromosome aberrations by human adenovirus type 12. Exp. Cell Res. 34: 400-403, 1964. PubMed: 14165598