CCD-8Lu (ATCC® CCL-201)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 48 years
Gender male
Ethnicity Caucasian
Applications
Derived from normal tissue from patient who died of cerebral thrombosis.
Karyotype normal human male; diploid; stable
Derivation
Derived from normal tissue from patient who died of cerebral thrombosis.
Clinical Data
Derived from normal tissue from patient who died of cerebral thrombosis.
male
Caucasian
48 years
Antigen Expression
blood type A; Rh +
Genes Expressed
blood type A; Rh +
Comments
Derived from normal tissue from patient who died of cerebral thrombosis.
The cells senesce at about population doubling level (PDL) 40 to 42.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 10,11
D5S818: 13
D7S820: 10,11
THO1: 7
TPOX: 8,9
vWA: 15,18
Population Doubling Time The cells senesce at about population doubling level (PDL) 40 to 42.
Name of Depositor GP Maxwell