R9ab (ATCC® CCL-193)

Organism: Oryctolagus cuniculus, rabbit  /  Cell Type: fibroblast  / 

Organism Oryctolagus cuniculus, rabbit
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age fetus
Gender female
Applications
Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
R9ab was derived from explants of biopsy material.
Karyotype normal; diploid; stable
Derivation
R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
Clinical Data
The donor indicated that the life expectancy of this line is at least 60 passages.
female
Comments
R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
The donor indicated that the life expectancy of this line is at least 60 passages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor EM Earley
Deposited As Oryctolagus cuniculus
Passage History
The donor indicated that the life expectancy of this line is at least 60 passages.
References

Castagliuolo I, et al. Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570

Basic Documentation
References

Castagliuolo I, et al. Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570