Don (ATCC® CCL-16)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 8 months
Gender male
Clinical Data
male
Virus Susceptibility Vesicular stomatitis virus
Human adenovirus 7
Human poliovirus 1 (attenuated)
Human poliovirus 3
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 1 to 2 minutes, remove trypsin and let culture stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37.0°C
Name of Depositor TC Hsu
Year of Origin September, 1962
References

J. Natl. Cancer Inst. 32: 857-869, 1964.

Cross References

Nucleotide (GenBank) : AF004368 Cricetulus griseus UDP-glucose pyrophosphorylase mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

J. Natl. Cancer Inst. 32: 857-869, 1964.