Gekko lung-1 (ATCC® CCL-111)

Organism: lizard, gecko (Gekko gecko), lizard, gecko  / 

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Organism lizard, gecko (Gekko gecko), lizard, gecko
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 38; range = 34 to 135.
Bimodal stemline number of 37 and 38 chromosomes. Stable pseudodiploid karyotype in 38 chromosome stemline number. The change from normal is a constant deletion in one of the largest chromosomes. Slightly unstable karyology in 37 chromosome stemline number.
Clinical Data
male
Virus Susceptibility Vaccinia virus
Vesicular stomatitis, Orsay (Indiana)
Human poliovirus 1
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 30°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Two to three times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 30.0°C
Name of Depositor HF Clark
Year of Origin June, 1965
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Cohen MM, Clark HF. Spontaneous chromosomal alteraion in cell lines of poikilothermic origin (Gekko gecko). Cytogenetics 7: 16-26, 1968. PubMed: 5659169

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Cohen MM, Clark HF. Spontaneous chromosomal alteraion in cell lines of poikilothermic origin (Gekko gecko). Cytogenetics 7: 16-26, 1968. PubMed: 5659169