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1.800.638.6597

BNL 1NG A.2 (ATCC^® TIB-76^™)

Organism: Mus musculus, mouse / Cell Type: chemically transformed / Tissue: liver / Disease: normal

BNL 1NG A.2 ATCC^® TIB-76^™

frozen

For-Profit: $551.00 Non-Profit: $459.15

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Permits and Restrictions	View Permits
Organism	Mus musculus, mouse
Tissue	liver
Cell Type	chemically transformed
Product Format	frozen
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	normal
Age	embryo
Strain	BALB/c
Applications	This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine. Tested and found negative for ectromelia virus (mousepox).

Derivation	This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine.
Tumorigenic	Yes
Effects	Yes, in BALB/c mice and ATxFL mice
Comments	This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine. The cells will grow in 0.3% agarose. Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Twice per week Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Name of Depositor	P Patek, J Collins, M Cohn
References	Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
References	Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934