Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390
Kimura S, et al. Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast. EMBO J. 6: 1929-1933, 1987. PubMed: 3308449
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.