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The c12 (B15ECiii2) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroylase (AHH) activity. The cells were exposed to ethyl methane sulfonate (EMS) and mutant colonies were selected for benzo[a]pyrene resistance. The c12 (B15ECiii2) cell line expresses reduced levels of aryl hydrocarbon receptor (AHR) mRNA and protein, due to an unknown factor required for AHR expression. AHR binds a number of xenobiotic compounds and mediates pathogenesis (including carcinogenesis) by these compounds. This cell line is a useful tool to study the molecular mechanism of these processes.
Hepa-1c1c7 cells were treated with ethylmethanesulfonate (EMS)