PLHC-1 (ATCC® CRL-2406)

Organism: Poeciliopsis lucida, topminnow  /  Cell Type: hepatocyte  /  Disease: hepatocellular carcinoma

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Organism Poeciliopsis lucida, topminnow
Cell Type hepatocyte
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatocellular carcinoma
Gender female
Applications
The PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cell line was derived from an adult female Poeciliopsis lucida, a topminnow from the Sonoran Desert.
These cells maintain a number of differentiated cell functions of hepatocytes.
PHLC-1 cells can be used in an in vitro system to screen environmentally relevant stressors such as heavy metals using a combined stress protein and cytotoxicity assay.
Increases in heat shock protein hsp70 can be detected in this system.
Derivation
The PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cell line was derived from an adult female Poeciliopsis lucida, a topminnow from the Sonoran Desert.
Clinical Data
The PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cell line was derived from an adult female Poeciliopsis lucida, a topminnow from the Sonoran Desert.
female
Receptor Expression
aryl hydrocarbon (Ah)
Genes Expressed
cytochrome P450 (CYP)
Comments
The PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cell line was derived from an adult female Poeciliopsis lucida, a topminnow from the Sonoran Desert.
A transplantable tumor was induced by multiple doses of 7, 12-dimethylbenz(a)anthracene (DMBA) treatment of the fish in 1982 by Mary E. Schultz.
The tumor was adapted to cell culture in 1985 by Lawrence E. Hightower.
These cells maintain a number of differentiated cell functions of hepatocytes. The cells possess inducible and stable cytochrome P450 (CYF) activity. This activity is inducible by 3,3',4,4'-tetrachlorobiphenyl (TCB).
Cytochrome P450 activity is also inducible by Aroclor 1254 (a chemical inducer of cytochrome P450-dependent monooxygenase activity), and reduced by EPN (an inhibitor of P450 activity).
PHLC-1 cells can be used in an in vitro system to screen environmentally relevant stressors such as heavy metals using a combined stress protein and cytotoxicity assay. Increases in heat shock protein hsp70 can be detected in this system.
Retention of hepatocyte properties has made this cell line ideal for in vitro toxicology assays; it has been well characterized by environmental toxicologists.
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 95%; fetal bovine serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Briefly rinse the cell layer with 0.05% Trypsin-0.53 mM EDTA solution. Add 2.0 to 3.0 ml additional Trypsin-EDTA solution to flask and allow flask to set at room temperature for 3 to 5 minutes until cells detach.
Tap flask and cells will slide off bottom of the flask.
Note: Cells come off in small clumps and are difficult to break up into single cells without greatly reducing cell viability.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 30.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor LE Hightower
References

Celander M, et al. Cytochromes P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1): dose- and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A. Arch. Biochem. Biophys. 329: 113-122, 1996. PubMed: 8619627

Huuskonen SE, et al. A fish hepatoma cell line (PLHC-1) as a tool to study cytotoxicity and CYP1A induction properties of cellulose and wood chip extracts. Chemosphere 36: 2921-2932, 1998. PubMed: 9734273

Schultz ME, Schultz RJ. Transplantable chemically-induced liver tumors in the viviparous fish Poeciliopsis. Exp. Mol. Pathol. 42: 320-330, 1985. PubMed: 2987024

Tocher DR, et al. Occurrence of 22:3n-9 and 22:4n-9 in the lipids of the topminnow (Poeciliopsis lucida) hepatic tumor cell line, PLHC-1. Lipids 30: 555-565, 1995. PubMed: 7651084

Fent K. Ecotoxicology of organotin compounds. Crit. Rev. Toxicol. 26: 1-117, 1996. PubMed: 8833456

Babich H, et al. In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida). Ecotoxicol. Environ. Saf. 21: 327-336, 1991. PubMed: 1868789

Ryan JA, Hightower LE. Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay. Environ. Tox. Chem. 13: 1231-1240, 1994.

Bruschweiler BJ, et al. Inhibitory effects of heavy metals on cytochrome P4501A induction in permanent fish hepatoma cells. Arch. Environ. Contam. Toxicol. 31: 475-482, 1996. PubMed: 8975819

Bruschweiler BJ, et al. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poecilopsis lucida). Aquat. Toxicol. 32: 143-160, 1995.

Hahn ME, et al. Cytochrome P4501A induction and inhibition by 3,3',4,4'-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1). Aquat. Toxicol. 26: 185-208, 1993.

Bruschweiler BJ, et al. Inhibition of cytochrome P4501A by organotins in fish hepatoma cells PCHC-1. Environ. Tox. Chem. 15: 728-735, 1996.

Bruschweiler BJ, et al. An ELISA asay for cytochrome P4501A in fish liver cells. Environ. Tox. Chem. 15: 592-596, 1996.

Hahn ME, et al. Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multiwell plates: response to TCDD, TCDF, and two planar PCBS. Environ. Tox. Chem. 15: 582-591, 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Celander M, et al. Cytochromes P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1): dose- and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A. Arch. Biochem. Biophys. 329: 113-122, 1996. PubMed: 8619627

Huuskonen SE, et al. A fish hepatoma cell line (PLHC-1) as a tool to study cytotoxicity and CYP1A induction properties of cellulose and wood chip extracts. Chemosphere 36: 2921-2932, 1998. PubMed: 9734273

Schultz ME, Schultz RJ. Transplantable chemically-induced liver tumors in the viviparous fish Poeciliopsis. Exp. Mol. Pathol. 42: 320-330, 1985. PubMed: 2987024

Tocher DR, et al. Occurrence of 22:3n-9 and 22:4n-9 in the lipids of the topminnow (Poeciliopsis lucida) hepatic tumor cell line, PLHC-1. Lipids 30: 555-565, 1995. PubMed: 7651084

Fent K. Ecotoxicology of organotin compounds. Crit. Rev. Toxicol. 26: 1-117, 1996. PubMed: 8833456

Babich H, et al. In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida). Ecotoxicol. Environ. Saf. 21: 327-336, 1991. PubMed: 1868789

Ryan JA, Hightower LE. Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay. Environ. Tox. Chem. 13: 1231-1240, 1994.

Bruschweiler BJ, et al. Inhibitory effects of heavy metals on cytochrome P4501A induction in permanent fish hepatoma cells. Arch. Environ. Contam. Toxicol. 31: 475-482, 1996. PubMed: 8975819

Bruschweiler BJ, et al. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poecilopsis lucida). Aquat. Toxicol. 32: 143-160, 1995.

Hahn ME, et al. Cytochrome P4501A induction and inhibition by 3,3',4,4'-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1). Aquat. Toxicol. 26: 185-208, 1993.

Bruschweiler BJ, et al. Inhibition of cytochrome P4501A by organotins in fish hepatoma cells PCHC-1. Environ. Tox. Chem. 15: 728-735, 1996.

Bruschweiler BJ, et al. An ELISA asay for cytochrome P4501A in fish liver cells. Environ. Tox. Chem. 15: 592-596, 1996.

Hahn ME, et al. Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multiwell plates: response to TCDD, TCDF, and two planar PCBS. Environ. Tox. Chem. 15: 582-591, 1996.