Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
- 10% fetal bovine serum
- 0.05 mM FUdR
- 0.1% pluronic F68
Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.