N1-S1 Fudr (ATCC® CRL-1603)

Organism: Rattus norvegicus, rat  /  Disease: hepatoma; Novikoff hepatoma

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Organism Rattus norvegicus, rat
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Disease hepatoma; Novikoff hepatoma
Gender male
Strain Sprague-Dawley
Applications
The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
Derivation
This line is derivative of N1-S1 (ATCC CRL-1604).
Clinical Data
male
Comments
The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
This line is derivative of N1-S1 (ATCC CRL-1604).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 10% fetal bovine serum
  • 0.05 mM FUdR
  • 0.1% pluronic F68

Subculturing
Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.
Name of Depositor JE Becker
References

Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488

Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488

Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803