H-4-II-E (ATCC® CRL-1548)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: hepatoma

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Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hepatoma
Applications
The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Genes Expressed
­
Cellular Products
aryl hydrocarbon hydroxylase is inducible
Comments
The line has been cured of Mycoplasma arginini contamination.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor JA Bradlaw
U.S. Patent Number
Product Origin http://prod-cms.atcc.org/sitecore/shell/Applications/Content%20editor.aspx#
References

Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894

. . In Vitro 16: 230, 1980.

Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181

Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233

Cross References

Nucleotide (GenBank) : U49128 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3b (delta part IVS4), partial cds.

Nucleotide (GenBank) : U49126 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta IVS4), partial cds.

Nucleotide (GenBank) : U49127 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta part IVS4), partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894

. . In Vitro 16: 230, 1980.

Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181

Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233