SK-RST (ATCC® CRL-2842)

Organism: Sus scrofa, pig  /  Tissue: kidney, cortex  /  Disease: normal

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Organism Sus scrofa, pig
Tissue kidney, cortex
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 1 day old newborn
Gender male
Applications The cells may be used in diagnostic tests for swine diseases and for production of products for use in veterinary and human medicine.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
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Derivation The cells were derived from the cortices of four kidneys obtained from two 1-day old pigs.
Clinical Data
male
Comments

The cells are free from the classical swine fever viruses and porcine circoviruses as described in 9 CFR 113.46, 9 CFR 113.47 and 9 CFR 113.55. 

The SK-RST cells exhibited cytopathology after being infected with two serotypes of vesicular stomatitis virus, Newcastle disease virus, porcine parvovirus, pseudorabies virus, reovirus, transmissible gastroenteritis virus, bovine virus diarrhea, bovine adenovirus, bovine parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and with representatives of the A, O, C, SAT1, SAT2, SAT3 and Asia serotypes of foot-and-mouth disease virus.

Classical swine fever virus (CSF)-infected SK-RST cells produced CSF antigen that was detectable using a CSF-antigen specific monoclonal antibody in an avidin-biotin complex immunohistochemistry assay. 

The SK-RST cells failed to exhibit cytopathology when exposed to porcine adenovirus, bluetongue virus, bovine respiratory syncytial virus, and bovine parvovirus. 

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when cells reach a concentration between 7 x 104 and 9 x 104 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:8 is recommended
Medium Renewal: Every two to three days.
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 21 hours
Name of Depositor RS Trowbridge
Year of Origin 1998
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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