ASK [Atlantic Salmon Kidney] (ATCC® CRL-2747)

Organism: Salmo salar, salmon, Atlantic  /  Cell Type: epithelial  /  Tissue: kidney  / 

Permits and Restrictions

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Organism Salmo salar, salmon, Atlantic
Tissue
kidney
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Applications
The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon.
The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.
Storage Conditions liquid nitrogen vapor phase
Derivation
­
Clinical Data
female
Comments
The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks.
Interval: Subculture every 10 to 17 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Once per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 20.0°C
Name of Depositor J Rolland, J Winton, B Krossoey
Year of Origin 1998
References

Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858