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NZP-60 (ATCC® CRL-1924™)

Organism: Mico melanura, black tailed marmoset  /  Tissue: kidney  /  Disease: normal

Permits and Restrictions

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Organism Mico melanura, black tailed marmoset
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Comments
Mico melanura or Mico melanurus (formerly named Callithrix argentata)
The cells can be propagated for at least 12 population doublings beyond frozen reference seed stock.
Complete Growth Medium

These cells are grown in Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12, 1:1 with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbonate, 15mM HEPES and 0.5mM sodium pyruvate.

Supplemented with:

  • 10% fetal bovine serum
  • 10 µg/ml insulin
  • 5.5 µg/ml transferrin
  • 5 ng/ml selenium
  • 40 ng/ml dexamethasone

This medium is formulated for use with a 5% CO2 in air atmosphere.

ATCC modified and tested medium formulation (without the additional supplements or serum described above) is available as ATCC 30-2006. 

ATCC tested fetal bovine serum is available as ATCC 30-2020 (500ml) and ATCC 30-2021 (100ml).
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor L Munson
Deposited As Callithrix argentata
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Restrictions

This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit