Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.