tk- ts13 (ATCC® CRL-1632)

Organism: Mesocricetus auratus, hamster, Syrian golden  / 

Organism Mesocricetus auratus, hamster, Syrian golden
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Applications
transfection host
Can be transfected at a high rate of efficiency.
Derivation
Thymidine kinase-deficient derivative of ts13, originally isolated as a temperature-sensitive mutant of the baby hamster kidney cell line BHK-21.
The tk-ts13 cell line is a derivative of BHK-21.
Comments
Thymidine kinase-deficient derivative of ts13, originally isolated as a temperature-sensitive mutant of the baby hamster kidney cell line BHK-21.
The tk-ts13 cell line is a derivative of BHK-21.
The cells are temperature sensitive for growth, and are thymidine kinase deficient (TK-).
The line is an efficient host for transfection studies.
The cells should be grown at 34C.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% versene solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks Inoculate new cultures at 2 X 10 exp4 cells/sq cm.
Culture Conditions
Temperature: 34.0°C
Name of Depositor R Baserga
References

Talavera A, Basilico C. Temperature sensitive mutants of BHK cells affected in cell cycle progression. J. Cell. Physiol. 92: 425-436, 1977. PubMed: 903382

Black ME, et al. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93: 3525-3529, 1996. PubMed: 8622970

Shen YM, et al. Superimposition of temperature regulation on yeast promoters. Mol. Cell. Biol. 2: 1145-1154, 1982. PubMed: 6294505

Basic Documentation
References

Talavera A, Basilico C. Temperature sensitive mutants of BHK cells affected in cell cycle progression. J. Cell. Physiol. 92: 425-436, 1977. PubMed: 903382

Black ME, et al. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93: 3525-3529, 1996. PubMed: 8622970

Shen YM, et al. Superimposition of temperature regulation on yeast promoters. Mol. Cell. Biol. 2: 1145-1154, 1982. PubMed: 6294505