G-402 (ATCC® CRL-1440)

Organism: Homo sapiens, human  /  Tissue: kidney  /  Disease: renal leiomyoblastoma

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease renal leiomyoblastoma
Age 9 months
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human cell line with the hyperdiploid chromosome count. The modal chromosome number was 47, occurring in 40% of cells. The rate of higher ploidies was 0.3%. The karyotype was 47,XX,+2,-5,+?ins(4) (q11.2) for the modal cell population. No other karyotypic abnormalities were evident.
Clinical Data
9 months
The line will grow in soft agar.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor PT Peebles
Deposited As Homo sapiens