LLC-MK2 Derivative (ATCC® CCL-7.1)

Organism: Macaca mulatta, monkey, rhesus  /  Tissue: kidney  / 

Organism Macaca mulatta, monkey, rhesus
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 70; range = 41 to 136.
No marker chromosomes noted.No marker chromosomes noted.
Genes Expressed
plasminogen activator; keratin.
Virus Susceptibility Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Comments

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor RN Hull
References

Holscher C, et al. Defective nitric oxide effector functions lead to extreme susceptibility of Trypanosoma cruzi-infected mice deficient in gamma interferon receptor or inducible nitric oxide synthase. Infect. Immun. 66: 1208-1215, 1998. PubMed: 9488415

Basic Documentation
References

Holscher C, et al. Defective nitric oxide effector functions lead to extreme susceptibility of Trypanosoma cruzi-infected mice deficient in gamma interferon receptor or inducible nitric oxide synthase. Infect. Immun. 66: 1208-1215, 1998. PubMed: 9488415