PK(15) (ATCC® CCL-33)

Organism: Sus scrofa, pig  /  Tissue: kidney  / 

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Organism Sus scrofa, pig
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Virus Susceptibility Classical swine fever virus , Classical swine fever virus
African swine fever virus
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Vaccinia virus
Human adenovirus 4
Human adenovirus 5
Human Coxsackievirus B 2
Human Coxsackievirus B3
Human Coxsackievirus B 4
Human Coxsackievirus B 5
Human Coxsackievirus B 6
Virus Resistance
poliovirus 2
Comments
The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC.

The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses.

The cell line harbors an endogenous C-type retrovirus.

The cells are positive for porcine circovirus (PCV) antigens.

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer twice with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor Cutter Laboratories, Inc.
References

Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830

Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860

Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256

Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097

Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060

Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403

James A House, personal communication

Cross References

Nucleotide (GenBank) : AJ133817 Porcine endogenous retrovirus type C proviral gag, pol and env genesand LTR (class A, clone 42).

Nucleotide (GenBank) : AJ133816 Porcine endogenous retrovirus type C proviral gag, pol and env genes and LTR (class B, clone 33).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830

Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860

Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256

Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097

Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060

Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403

James A House, personal communication