LLC-RK1 (ATCC® CCL-106)

Organism: Oryctolagus cuniculus, rabbit  /  Tissue: kidney  /  Disease: normal

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Organism Oryctolagus cuniculus, rabbit
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
Plasminogen activator; keratin
Virus Resistance
poliovirus 1
Comments

The cells are positive for keratin by immunoperoxidase staining.

The cells are negative for bovine viral diarrhea virus (BVDV).

Electron microscopic studies show many bulb gap junctions (BGJ).

Complete Growth Medium Medium 199 with 1.12 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
  5. Discard supernatant and resuspend cells in fresh growth medium Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

    Culture Conditions
    Temperature: 37°C
    Name of Depositor RN Hull
    References

    Hull RN, et al. Development and characteristics of the rabbit kidney cell line strain, LLC-RK. Proc. Soc. Exp. Biol. Med. 118: 1054-1059, 1965. PubMed: 14277663

    Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

    Hull RN, Butorac G. The utility of rabbit kidney cell strain, LLC-RK to rubella virus studies. Am. J. Epidemiol. 83: 509-517, 1966. PubMed: 4956596

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Hull RN, et al. Development and characteristics of the rabbit kidney cell line strain, LLC-RK. Proc. Soc. Exp. Biol. Med. 118: 1054-1059, 1965. PubMed: 14277663

    Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

    Hull RN, Butorac G. The utility of rabbit kidney cell strain, LLC-RK to rubella virus studies. Am. J. Epidemiol. 83: 509-517, 1966. PubMed: 4956596

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.