Complete Growth Medium
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The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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Subculturing
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Cultures can be maintained by addition of fresh medium or
replacement of medium. Alternatively, cultures can be
established by centrifugation with subsequent resuspension in fresh medium.
Add fresh medium as cell density increases.
Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Every 3 to 5 days
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Cryopreservation
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Culture medium, 95%; DMSO, 5%
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