HRT-18G (ATCC® CRL-11663)

Organism: Homo sapiens, human  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 67 years
Gender male
Applications
They can be used for propagation and plaque titration of bovine coronaviruses.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of HRT-18 and represents a subpopulation of cells selected by successive passage in different media.
Clinical Data
male
Comments
This line is a derivative of HRT-18 (ATCC CCL-244) and represents a subpopulation of cells selected by successive passage in different media.
HRT-18G cells show enhanced susceptibility to bovine coronaviruses relative to the parental HRT-18 cell line.
They can be used for propagation and plaque titration of bovine coronaviruses.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Cryopreservation
culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor Louisiana State Univ. Agricultural and Mechanical College
U.S. Patent Number
Passage History
This line is a derivative of HRT-18 and represents a subpopulation of cells selected by successive passage in different media.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation