LS 180 (ATCC® CL-187)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: Dukes' type B, colorectal adenocarcinoma

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Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type B, colorectal adenocarcinoma
Age 58 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 45; range = 42 to 47.
The stemline karyotype was 45,t(X;5) (q12;q31) with tetraploid-like cells occurring at 2.2 percent. Occasional changes in karyotypes were also noted as a result of balanced translocations producing new marker chromosomes. In conjunction with this, decreases in chromosomes involving interchromosomal exchanges were seen. The karyotype was exactly the same as that originally described by the depositor.
Clinical Data
female
58 years
Caucasian
Antigen Expression
serologically defined colon cancer antigen 3; Homo sapiens, expressed
HLA A2, B13, B50; Blood type O
Genes Expressed

carcinoembryonic antigen (CEA), interleukin 10, interleukin 6, mucin

The production of CEA in the ATCC seed stock was 1916 ng per 10(6) cells in 10 days. 
 

Tumorigenic Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells
Effects
Yes, in nude mice
Comments
This line has never been trypsinized; however, the variant LS 174T (ATCC CL-188) has been subcultured by trypsinization.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove most medium. Dislodge cells from the floor of the flask by banging flask, washing cells by repeat pipetting and scraping gently. Aspirate and dispense into new flasks. These cells have never been trypsinized.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C

STR Profile
Amelogenin: X
CSF1PO: 10,13
D13S317: 10
D16S539: 11,13
D5S818: 10,15
D7S820: 11,9.3
THO1: 6,7
TPOX: 8,9
vWA: 15,18
Isoenzymes
ADA, 1
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Name of Depositor Northwestern University
References

Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041

Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Schuetz EG, et al. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc. Natl. Acad. Sci. USA 93: 4001-4005, 1996. PubMed: 8633005

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041

Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Schuetz EG, et al. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc. Natl. Acad. Sci. USA 93: 4001-4005, 1996. PubMed: 8633005