SW756 (ATCC® CRL-10302)

Organism: Homo sapiens, human  /  Disease: squamous cell carcinoma

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 76; range = 60 to 96
Derivation
Temple Texas, United States
Clinical Data
female
Caucasian
46 years
Antigen Expression
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Genes Expressed
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Tumorigenic Yes
Effects
Yes, in nude mice
Virus Susceptibility Influenza A virus , Influenza A virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17
Isoenzymes
G6PD, B
GLO-I, 1-2
Name of Depositor Univ. Texas System, Board of Regents
Year of Origin 1974
References

Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

PR8/A/34

The SW756 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1974 from biopsy material from a poorly differentiated invasive squamous carcinoma of the uterine cervix. The patient had not received chemotherapy or radiation prior to the biopsy.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

PR8/A/34

The SW756 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1974 from biopsy material from a poorly differentiated invasive squamous carcinoma of the uterine cervix. The patient had not received chemotherapy or radiation prior to the biopsy.