MDA-MB-330 (ATCC® HTB-127)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 43 years
Gender female
Ethnicity Caucasian
Applications
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Karyotype modal number = 64 with one marker chromosome
Images
Clinical Data
female
Caucasian
43 years
Antigen Expression
Blood Type A; Rh+
Receptor Expression
epidermal growth factor (EGF)
Cellular Products
tubulin; actin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Most cells of the line stained positively for actin.
This line grows very slowly (doubling time is 10 to 20 days) and never becomes confluent.
Complete Growth Medium These cells are grown in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with:
  • 30 ng/ ml human recombinant EGF
  • 0.016 mg/ml human recombinant insulin
  • 2mM glutathione
  • 20% fetal bovine serum
Do not filter EGF thru 0.22 micron filter.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Once per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 9,12
D5S818: 13
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Population Doubling Time 240 to 480 hrs
Name of Depositor R Cailleau
References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

Unless the Purchaser has a separate license agreement with The University of Texas M. D. Anderson Cancer Center (“Institution”), the ATCC Material is subject to the following restrictions:

  1. The ATCC Material (and any Modifications, Unmodified Derivatives and/or Progeny thereof) may not be used (1) for commercial purposes or Commercial Use by any Purchaser, or (2) by for-profit or commercial entities for any purpose, provided, however, that ATCC Material that is purchased from ATCC as deoxyribonucleic acid and/or ribonucleic acid or nucleotide chains of any length (hereinafter, “Nucleic Acids”) may be used for internal research purposes by any Purchaser of Nucleic Acids;
  2. Purchaser may not transfer ATCC Materials, Modifications, Unmodified Derivatives, or Progeny to any for-profit entity or commercial entity; and
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References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202