NG108-15 [108CC15] (ATCC® HB-12317)

Organism: Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma)  /  Cell Type: somatic cell hybrid  /  Tissue: brain  /  Disease: glioblastoma; neuroblastoma

Organism Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma)
Tissue brain
Cell Type somatic cell hybrid
Product Format frozen
Morphology flat; round; 10 to 100 micrometers diameter
Culture Properties Adherent, but please note: as the culture media becomes acidic these cells begin to detach and grow as a suspension, but they will typically reattach again when fresh medium is added.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease glioblastoma; neuroblastoma
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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Derivation
The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus.
The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht.
Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
  • 0.1 mM hypoxanthine (final conc.)
  • 400 nM aminopterin (final conc.)
  • 0.016 mM thymidine (final conc.)
  • 10% fetal bovine serum (final conc.)
  • 1.5 g/L sodium bicarbonate

Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor Univ. Texas Southwestern Medical Cntr.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920

Basic Documentation
Other Documentation
References

Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920