NCI-H1238 [H1238] (ATCC® CRL-5859)

Organism: Homo sapiens, human  /  Disease: stage E, carcinoma; small cell lung cancer (SCLC)

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Culture Properties suspension; some adherent cells
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease stage E, carcinoma; small cell lung cancer (SCLC)
Age 64 years
Gender male
Ethnicity White
The line was established in November 1985.
Clinical Data
The patient was a smoker.
64 years
The line was established in November 1985.
The patient was a smoker.
130 pack years.
Complete Growth Medium HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Some cells will attach.
Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 10
D5S818: 13
D7S820: 11
THO1: 9,9.3
vWA: 18,19
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1985

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI).  For further information, please contact NCI’s Technology Transfer Center at or by phone at (240)-276-5514.


NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.