SK-N-BE(2) (ATCC® CRL-2271)

Organism: Homo sapiens, human  /  Cell Type: neuroblast, Neuroblastoma  /  Tissue: brain; derived from metastatic site: bone marrow  /  Disease: neuroblastoma

Permits and Restrictions

View Restrictions

Organism Homo sapiens, human
Tissue brain; derived from metastatic site: bone marrow
Cell Type neuroblast, Neuroblastoma
Product Format frozen
Morphology neuroblast
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease neuroblastoma
Age 2 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 44; one or more HSR bearing chromosomes
Derivation
The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Clinical Data
male
2 years
Tumorigenic Yes
Effects
Yes, an inoculum of 107 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency
Comments
The cells exhibit moderate levels of dopamine beta hydroxylase activity.
SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2.
The morphology of the cells varies with some cells having long processes and others that are epithelioid like.
The cells will aggregate, form clumps and float.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with a fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:12 to 1:20 is recommended.
Medium Renewal: Every 4 to 7 days.
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6,7
TPOX: 8,11
vWA: 18
Population Doubling Time 30 hrs
Name of Depositor JL Biedler
Year of Origin November, 1972
References

Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PubMed: 62055

Barnes EN, et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK- N-BE(2), and SK-N-MC. In Vitro 17: 619-131, 1981. PubMed: 7327593

Biedler JL, Spengler BA. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-187, 1976. PubMed: 942798

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704

Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PubMed: 62055

Barnes EN, et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK- N-BE(2), and SK-N-MC. In Vitro 17: 619-131, 1981. PubMed: 7327593

Biedler JL, Spengler BA. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-187, 1976. PubMed: 942798

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704