7F2 (ATCC® CRL-12557)

Organism: Mus musculus, mouse  /  Cell Type: osteoblast  /  Tissue: bone marrow  / 

Organism Mus musculus, mouse
Tissue bone marrow
Cell Type osteoblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 5 to 6 weeks
Gender male
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
7F2 is a spontaneously immortalized cell line isolated from p53-/- mouse bone marrow taken from the femur and subsequently cloned. (Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998)
Clinical Data
male
Genes Expressed
alkaline phosphatase
Comments

The cells appeared to correspond to mature osteoblasts because they express alkaline phosphatase, secrete type I collagen, show a significant cyclic adenosine monophosphate response to parathyroid hormone, secrete osteocalcin, and mineralize extensively. However, in contrast to mature osteoblasts, this clone can be induced to undergo massive adipocyte differentiation, and this differentiation is accompanied by the complete loss of expression of all osteoblastic markers except alkaline phosphatase. These observations indicate that some cells that have acquired all of the characteristics of mature osteoblasts can be diverted to the adipocyte pathway. RefThompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512

Complete Growth Medium Alpha minimum essential medium with 2 mM l-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor ZymoGenetics, Inc.
References

Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998

Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512

Basic Documentation
Other Documentation
References

Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998

Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512