FDC-P1 (ATCC® CRL-12103)

Organism: Mus musculus, mouse  /  Tissue: bone marrow  /  Disease: Normal

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Organism Mus musculus, mouse
Tissue bone marrow
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Strain DBA/2
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor temperature
Karyotype diploid
Derivation
The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).
Antigen Expression
Thy-1.2 (24%)
Comments
They are dependent upon WEHI-3 conditioned medium (WEHI-3CM) for continued growth.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  To make the complete growth medium, add the following components to the base medium:  
  • mouse Interleukin-3 culture supplement (BD Biosciences, Catalog No. 354040)  25% (final conc.)
  • fetal bovine serum  10% (final conc.)
To make a 500 mL volume:
  • 325 mL DMEM (ATCC 30-2002)
  • 125 mL Mouse Interleukin-3 (BD 354040)
  • 50 mL FBS (ATCC 30-2020)
This medium is formulated for use with a 5% CO2 in air atmosphere. Standard DMEM formulations contain 3.7 g/L sodium bicarbonate.  A 10% CO2 in air atmosphere is recommended.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor University of Virginia Patent Foundation
U.S. Patent Number
References

Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334