RD-ES (ATCC® HTB-166)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: Ewing's sarcoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology epithelial
Culture Properties mixed: adherent and clusters in suspension
Biosafety Level 1
Disease Ewing's sarcoma
Age 19 years
Gender male
Ethnicity Caucasian
Derivation
The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.
Clinical Data
19 years
Caucasian
male
Antigen Expression

Blood type B; Rh+

Comments
Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.
The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1-2
PGM3, 1
Name of Depositor G Marshall, M Kirchen
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation