Complete Growth Medium
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The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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Subculturing
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Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5-8 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
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Cryopreservation
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Culture medium, 95%; DMSO, 5%
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