E.H. IV (Elaine IV) (ATCC® CCL-104)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 20 years
Gender female
Ethnicity Caucasian
Applications
The cells carry and produce Epstein-Barr virus (EBV).
Clinical Data
female
Caucasian
20 years
Genes Expressed
Epstein-Barr virus (EBV)
Cellular Products
Epstein-Barr virus (EBV)
Virus Resistance
partially resistant to poliovirus and vesicular stomatitis viruses
Comments
The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Biological Safety CLASS II.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.
Isoenzymes
G6PD, B
Name of Depositor W Henle
References

Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA 203: 205-209, 1968. PubMed: 4864269

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA 203: 205-209, 1968. PubMed: 4864269