MTKP 97-12 (ATCC® CRL-8985)

Organism: Mus musculus, mouse  /  Cell Type: Melanoma  / 

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Organism Mus musculus, mouse
Cell Type Melanoma
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 100 days
Gender male
Strain C3H/An
Applications
produces protein antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Derivation
The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen.
Clinical Data
male
Genes Expressed
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 [MFI2],antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Comments
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3.
The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen.
The cells produce approximately 4,000,000 molecules of p97 per cell under the control of the mouse metallothionein promoter.
A metallothionein promoter has been added upstream of the cDNA sequence. Expression can be induced by treating with 80 uM ZnCl and 9 uM CdCl for 24 hrs.
Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300.
Complete Growth Medium Dulbecco's modified Eagle's medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Twice per week
Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium, 50%; fetal bovine serum, 40%; DMSO, 10%
Name of Depositor Oncogen
References

Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992

Brian W Poor, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992

Brian W Poor, personal communication