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Ltk-11 (ATCC® CRL-10422™)

Organism: Mus musculus, mouse  / 

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 100 days adult
Gender male
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Clinical Data
male
Receptor Expression
5-hydroxytryptamine (serotonin) 1D beta (5HT1Dbeta)
Comments
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor Synaptic Pharmaceutical Corporation
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004